Device
Part:BBa_K766007:Design
Designed by: Yu Zhao Group: iGEM12_Tsinghua (2012-09-24)
Plas and cI hybrid promoter with RFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 57
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 744
Illegal AgeI site found at 856 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We want to design a part that can be activated by one signal but repressed by another signal.
Source
We hybrid Plas promoter and cI repressor by overlap PCR and add RFP downstream by another step of overlap PCR.